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Apr 2023;26 Suppl 1:31-46

Cell viability was determined using the Cell Counting Kit-8 (Dojindo, Japan) as described previously (8)

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Moreover, although one can expect that elevated homocysteine levels result from the vascular disease per se (Kuller and Evans, 1998), the cardiovascular risk is gradually related to plasma homocysteine in many studies (Hopkins et al, 1995